Services

Services

Services provided by the genomics core facility include DNA and RNA quantification, chemical and mechanical fragmentation, NextGen sequencing library preparation and normalization, whole genome de novo and resequencing, whole transcriptome sequencing (RNASeq), and whole exome sequencing. We also design specialized library prep and seq methods such as whole genome methylation analysis and targeted region of interest sequencing. Please contact the Genomics Center manager, Taegun Kwon regarding services and sample submission.

Next Gen Sequencing Services

NEXTSEQ 500 SYSTEM
The NextSeq 500 is the right sequencer for any project size. It is the only desktop sequencing system capable of sequencing a high coverage (30x) whole human genome in one run. Its flexible throughput means no waiting to fill a flowcell and thus a quicker turnaround time for your sequencing sample. Because of high and mid output options, the NextSeq can easily shift between high and low throughput projects
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MISEQ 2 SYSTEM
The MiSeq 2 desktop sequencer allows for more focused applications such as targeted gene sequencing, metagenomics, small genome and transcriptome sequencing, targeted gene expression, and amplicon sequencing. Generating up to 25 million reads, the MiSeq sequencer is ideal for reduced representation studies and narrowly focused applications.
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NANODROP ONE SPECTROPHOTOMETER
Designed to work specifically with downstream applications such as NGS projects, the NanoDrop One assesses sample contaminants and reports the adjusted sample quantity accordingly.
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QUBIT 3.0 FLUOROMETER
The Qubit has the ability to differentiate between dsDNA, ssDNA, RNA, and protein. Concentration is equal to this signal of fluorescent dye emitted only when the dye binds to the sample being assayed. Most contaminants have no effect on the final result. This makes the Qubit extremely useful when accurate quantitation is needed for downstream applications.
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AGILENT 2100 BIOANALYZER SYSTEM
The Agilent 2100 Bioanalyzer system analyzes total RNA, small and micro RNAs, and varying sizes of DNA fragments. This chip-based capillary electrophoresis has a low sample consumption of 1┬ÁL providing the GC with an effective method of determining fragment size distributions. However, the Bioanalyzer is not equipped for accurate quantification of concentration.This device serves as the indicator of whether a sample can continue for further sequencing.
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AGILENT 4200 TAPESTATION SYSTEM
The Agilent 4200 TapeStation system is fully automated and designed specifically to support Next Generation Sequencing workflows. Many sample types can be analyzed on the TapeStation, including total RNA, labeled RNA, small and micro RNAs, as well as small and large DNA fragments.The most notable improvement this technology brings to our facility is its scalability. Even when investigating the quality of a single sample, the TapeStation allows for a constant cost. No longer does a lab have to waste funds because it is forced to pay in 12 sample increments.
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