Services

Metabolomics is the study of small-molecules (metabolites) of a biological organism, with the metabolome representing the collection of all metabolites.

BAF focuses on the detection and quantitation of selected small molecules (mass-to-charge ratio [m/z] up to 2,250) from a biological source. Our methods are designed to take advantage of state-of-the art liquid chromatographers coupled to highly sensitive mass spectrometers (AB Sciex QTRAP 6500+, QTRAP 7500 , and TripleTOF 6600+). Moreover, the laboratory has a gas chromatographer mass spectrometer systems (Thermo ISQ1310 and Agilent 5975B single quadrupoles) capable of performing liquid, headspace or solid phase micro-extraction injections, and identify new biomarkers using NIST17. The BAF can detect and quantify over 400 metabolites including sugars, sugar alcohols, amino acids, organic acids, phosphorylated compounds, polyamines, phenolics, hormones, secondary metabolites, fatty acids, and polyunsaturated aldehydes.

In addition, the staff can design and validate methods for new metabolites that clients want to pursue.

For information regarding analytical projects, sample submission, instrument training, and use, contact Jean Christophe Cocuron (Jeanchristophe.Cocuron@unt.edu).


LC-MS/MS
1. TARGETED METABOLOMICS
Quantitative analysis of key metabolite classes

  • Phosphorylated compounds
  • Amino acids
  • Organic acids
  • Polyphenols
  • Sugars
LC-MS/MS
2. TARGETED LIPIDOMICS
Quantitative profiling of lipid classes and molecular species

  • Glycerolipids
  • Phospholipids
  • Galactolipids
  • Sphingolipids
  • Cholesteryl esters
LC-HR-MS/MS
3. UNTARGETED METABOLOMICS
Broad-spectrum metabolite discovery and profiling

  • In-house library
  • MoNA
  • HMDA
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GC-MS

Wiley/NIST Spectral Library 2023

LOOKING FOR SOMETHING NOT LISTED?

Our capabilities extend beyond the metabolites listed above.
Our staff can develop and validate new analytical methods to support additional metabolites and specific reserach needs.